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Super-resolution measurement of distance between transcription sites using RNA FISH with intronic probes
Nascent transcripts being copied from specific human genes can be detected using RNA FISH (fluorescence in situ hybridization) with intronic probes, and the distance between two different nascent transcripts is often ...
Binding of nuclear factor kappa B to noncanonical consensus sites reveals its multimodal role during the early inflammatory response
Mammalian cells have developed intricate mechanisms to interpret, integrate, and respond to extracellular stimuli. For example, tumor necrosis factor (TNF) rapidly activates proinflammatory genes, but our understanding of ...
Preparation of photoluminescence tunable Cu-doped AgInS2 and AgInS2/ZnS nanocrystals and their application as cellular imaging probes
In this work, high-quality Cu doped AIS and AIS/ZnS NCs have been first synthesized via a surface doping approach. By varying the Cu doping concentrations, Cu doped AIS NCs exhibit a photoluminescence red-shift from around ...
BioSig3D: High Content Screening of Three-Dimensional Cell Culture Models
BioSig3D is a computational platform for high-content screening of three-dimensional (3D) cell culture models that are imaged in full 3D volume. It provides an end-to-end solution for designing high content screening assays, ...
Stiffness of the microenvironment upregulates ERBB2 expression in 3D cultures of MCF10A within the range of mammographic density
The effects of the stiffness of the microenvironment on the molecular response of 3D colony organization, at the maximum level of mammographic density (MD), are investigated. Phenotypic profiling reveals that 3D colony ...
Quality control of subgrade soil using intelligent compaction
Intelligent Compaction (IC) of subgrade soil has been proposed to continuously monitor the stiffness of subgrade during its compaction. Modern IC rollers are vibratory compactors equipped with (1) an onboard measuring ...