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Functionalized Buckyballs for Visualizing Microbial Species in Different States and Environments
(Scientific Reports, 2015)
To date, in situ visualization of microbial density has remained an open problem. Here, functionalized buckyballs (e.g., C60-pyrrolidine tris acid) are shown to be a versatile platform that allows internalization within a ...
Buckyballs conjugated with nucleic acid sequences identifies microorganisms in live cell assays
Rapid identification of bacteria can play an important role at the point of care, evaluating the health of the ecosystem, and discovering spatiotemporal distributions of a bacterial community. We introduce a method for ...
High-Dimensional Phenotyping Identifies Age-Emergent Cells in Human Mammary Epithelia
Aging is associated with tissue-level changes in cellular composition that are correlated with increased susceptibility to disease. Aging human mammary tissue shows skewed progenitor cell potency, resulting in diminished ...
Fusion of encoder-decoder deep networks improves delineation of multiple nuclear phenotypes
Nuclear segmentation is an important step for profiling aberrant regions of histology sections. If nuclear segmentation can be resolved, then new biomarkers of nuclear phenotypes and their organization can be predicted for ...
BioSig3D: High Content Screening of Three-Dimensional Cell Culture Models
BioSig3D is a computational platform for high-content screening of three-dimensional (3D) cell culture models that are imaged in full 3D volume. It provides an end-to-end solution for designing high content screening assays, ...
Stiffness of the microenvironment upregulates ERBB2 expression in 3D cultures of MCF10A within the range of mammographic density
The effects of the stiffness of the microenvironment on the molecular response of 3D colony organization, at the maximum level of mammographic density (MD), are investigated. Phenotypic profiling reveals that 3D colony ...