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Effects of asprosin on steroidogeneses of bovine granulosa cells
Date
2020Type
ThesisDepartment
Animal Science
Degree Level
Master's Degree
Abstract
Asprosin is a novel hormone associated with disorders, such as insulin resistance andpolycystic ovarian syndrome. Understanding ovarian steroidogenesis is imperative to
understand how health and fertility could be managed. Although, asprosin impacts
steroidogenesis of ovarian theca cells, its impacts on granulosa cells (GC) remains to be
unveiled. Hence, the objective of this study was to investigate asprosin impacts on GC
steroidogenesis. Bovine GC from small follicles of ovaries were used to investigate cell
proliferation, estradiol production, and mRNA abundance of steroidogenic enzymes such
as steroidogenic acute regulatory protein (StAR), cholesterol side-chain cleavage enzyme
(CYP11A1), and aromatase (CYP19A1). Asprosin enhanced FSH-induced GC production
of estradiol, but diminished IGF1-induced estradiol synthesis in comparison to IGF-1
alone. Asprosin decreased GC proliferation induced by IGF-1 alone or in combination
with FSH. Asprosin increased CYP11A1 mRNA abundance when combined with FSHtreated or IGF-1-treated GC. Taken together, the present findings indicate that asprosin
regulates GC steroidogenesis and proliferation and suggests that asprosin is a promising
ovarian steroidogenic and folliculogenesis regulator. Further studies are required to
unveil how asprosin affects GC function and its importance to fertility and health.
Permanent link
http://hdl.handle.net/11714/7728Subject
Asprosincattle
ovarian steroidogenesis
Additional Information
Committee Member | Fonseca, Mozart; Ferguson, Bradley S |
---|---|
Rights | Creative Commons Attribution 4.0 United States |
Rights Holder | Author(s) |