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Good Vibrations: Reliable Vibrational Probes for Investigating Structure and Dynamics in Biomolecules
AdvisorTucker, Matthew J.
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Monitoring changes in the local electrostatic environment of proteins is important for determining the dynamic nature involved in many biological processes. The efficacy of natural and non-natural vibrational probes has been tested for monitoring the conformational dynamics in peptide and protein systems. Vibrational probe pairs can simultaneously detect dynamics at two different regions of the biomolecules active site via 2D IR. The variations in the distance and orientation of a probe-pair provide the details of molecular movements which affects the protein function. Various probe-pair have been developed and incorporated for structural determination. Spectroscopic rulers can be obtained by utilizing these probe-pairs with detecting the changes in coupling strength by variation in distance within protein systems.