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Mitochondrial haplotypes are not associated with mice selectively bred for high voluntary wheel running
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Mitochondrial haplotypes have been associated with human and rodent phenotypes, including nonshiveringthermogenesis capacity, learning capability, and disease risk. Although the mammalian mitochondrial D-loop ishighly polymorphic, D-loops in laboratory mice are identical, and variation occurs elsewhere mainly betweennucleotides 9820 and 9830. Part of this region codes for thetRNAArggene and is associated with mitochondrialdensities and number of mtDNA copies. We hypothesized that the capacity for high levels of voluntary wheel-running behavior would be associated with mitochondrial haplotype. Here, we analyzed the mtDNA poly-morphic region in mice from each of four replicate lines selectively bred for 54 generations for high voluntarywheel running (HR) and from four control lines (Control) randomly bred for 54 generations. Sequencing thepolymorphic region revealed a variable number of adenine repeats. Single nucleotide polymorphisms (SNPs)varied from 2 to 3 adenine insertions, resulting in three haplotypes. We found significant genetic differentiationsbetween the HR and Control groups (Fst= 0.779,p?0.0001), as well as among the replicate lines of micewithin groups (Fsc= 0.757,p?0.0001). Haplotypes, however, were not strongly associated with voluntarywheel running (revolutions run per day), nor with either body mass or litter size. This system provides a usefulexperimental model to dissect the physiological processes linking mitochondrial, genomic SNPs, epigenetics, ornuclear-mitochondrial cross-talk to exercise activity.
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