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Potential Diagnostic Power of Blood Circular RNA Expression in Active Pulmonary Tuberculosis
Author
Qian, ZhongqingLiu, Hui
Li, Musheng
Shi, Junchao
Li, Na
Zhang, Yao
Zhang, Xiaojie
Lv, Jingzhu
Xie, Xueying
Bai, Yunfei
Ge, Qinyu
Ko, Eun-A.
Tang, Haiyang
Wang, Ting
Wang, Xiaojing
Wang, Zhaohua
Zhou, Tong
Gu, Wanjun
Date
2018Type
ArticleAbstract
Circular RNAs (circRNAs) are a class of novel RNAswith important biological functions, and aberrant expression of circRNAs has been implicated in human diseases. However, the feasibility of using blood circRNAs as disease biomarkers is largely unknown. Methods: We explored the potential of using human peripheral blood mononuclear cell (PBMC) circRNAs as marker molecules to diagnose active pulmonary tuberculosis (TB). Findings: First, we demonstrated that circRNAs are widely expressed in human PBMCs and that many are abundant enough to be detected. Second, we found that the magnitude of PBMC circRNAs in TB patients was higher than that in the paired healthy controls. Comparedwith host linear transcripts, the circRNAs with in several pathways are disproportionately upregulated in active TB patients, including "Cytokine-cytokine receptor interaction", "Chemokine signaling pathway", "Neurotrophin signaling pathway", and "Bacterial invasion of epithelial cells". Based on the differentially expressed circRNAs within these pathways, we developed a PBMC circRNAbasedmolecular signature differentiating active TB patients fromhealthy controls. We validated the classification power of the PBMC circRNA signature in an independent cohort with the area under the receiver operating characteristic curve (AUC) at 0.946. Interpretation: Our results suggest that PBMC circRNAs are potentially reliable marker molecules in TB diagnosis. (c) 2017 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license.
Permanent link
http://hdl.handle.net/11714/5506Subject
Circular RNATuberculosis
Biomarker
PBMC
Additional Information
Journal Title | EBioMedicine |
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Rights | Creative Commons Attribution-NonCommercial-NoDerivatives- 4.0 International |
Rights Holder | Authors |