Analysis of Anterior Lateral Mechanosensory (ALM) neuron migration in srf-8 (dv38) Caenorhabditis elegans Mutants
AuthorWeatherly, Christopher Scott
AdvisorBerninsone, Patricia M.
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Proper neuron migration is regulated by a plethora of modulatory proteins, sugars, and other molecules. These complex mechanisms can be studied on an organismal level in the simple nematode C. elegans. The C. elegans mutant srf-8 (dv38) carries a known intracellular mutation in a p24 protein which is involved in cargo selectivity of molecules transported from the endoplasmic reticulum to the Golgi apparatus. Disruption of this process effects protein transport and cell-surface glycosylation, which has been shown to disturb neuron migration. These mutant worms show defective migration in a wide variety of neuron and cell migrations, notably Anterior Lateral Mechanosensory (ALM) neuron migration. Previous literature illustrates that other C. elegans mutants [sdn-1 (zh20), slt-1 (eh15), sax-3 (ky123), sax-3 (ky200), unc-40 (e271)] are known to have defects in ALM migration that are exclusive from the p24 protein. In order to visualize the ALM neuron, we used a mec-4