Separation and Purification of a Monoclonal Antibody for use in Patients Suffering from Wet Macular Degeneration
Del Bianco, Marcus
Henrique Israel, Luis
AdvisorFuchs, Alan W.
Chemical and Materials Engineering
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Novel process designs were created in an effort to optimize the efficiency of monoclonal antibody separation and purification for an industrial process. A base case was established that utilized common protein separation techniques such as cation exchange chromatography, anion exchange chromatography, and hydrophobic interaction chromatography (HIC). Alternate process designs were also made that utilized immobilized metal affinity chromatography (IMAC) and various multimodal chromatography mediums. Experiments were conducted to determine the most effective mediums that could be used in the process design. To better analyze the new alternate plans, an economic analysis was conducted which determined what alternate plan would be more cost effective. From analysis, alternate design 1 and alternate design 2a were determined to be the preferred design due to enhanced performance and lesser overall capital cost.