Use of Saliva Biomarkers to Monitor Efficacy of Vitamin C in Exercise-induced Oxidative Stress
AuthorEvans, Levi Wesley
AdvisorOmaye, Stanley T.
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Saliva is an appealing biospecimen and can be easily obtained for health research. A popular discussion in health research is oxidative stress. Systemic biomarkers that represent oxidative stress and antioxidant status such as malondialdehyde and vitamin C can be found in saliva. It is unclear, however, if saliva is an accurate biospecimen as is blood and plasma for systemically monitoring such biomarkers. Exercise can induce oxidative stress, resulting in a trend of antioxidant supplementation to combat its assumed detriments. Vitamin C is a popular antioxidant supplement in the realm of sports and exercise but its role in the area is unconfirmed. One potential avenue for evaluating exercise-induced oxidative stress is through assessment of biomarkers like vitamin C, thiobarbituric acid reactive substances (TBARS), and malondialdehyde (MDA) in saliva. Salivary vitamin C was examined before and after a vitamin C supplementation period of four weeks. Oxidative stress was induced via resistance exercise. Free malondialdehyde and TBARS were used as the salivary biomarkers to assess oxidative stress before and after exercise and vitamin C supplementation. Peak muscular force was measured before and after the supplementation period to examine any exercise performance enhancement. Salivary vitamin C increased after the supplementation period along with post-resistance exercise. Free malondialdehyde in saliva increased after resistance exercise and was reduced after the supplementation period; similar results have been experienced with systemic biomarker examination. An increase in peak muscular force was experienced after the supplementation period. Saliva could potentially be used to assess vitamin C status and exercise-induced oxidative stress. Further, vitamin C may be a potentially beneficial supplement for resistance exercise and warrants further research.