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Thesis Advisor | Baker, Josh E. | |
Author | Latham-Kapitz, Madison | |
Date Accessioned | 2017-08-31T21:17:43Z | |
Date Available | 2017-08-31T21:17:43Z | |
Date of Issue | 2017 | |
Identifier (URI) | http://hdl.handle.net/11714/1923 | |
Description | When skeletal and cardiac muscle contract they generate movement through the actin-myosin ATPase reaction. However, the chemical reaction underlying movement remains unclear. Specifically, little is known about how the forces generated by muscle influence the chemistry of the force-generating reaction. We measured the effects of mechanical forces on the actin-myosin ATPase activity using a standard motility assay and analysis for skeletal and cardiac muscle myosin. A mechanical load was imposed on a sliding actin filaments using alpha-actinin, and the corresponding effects of load were determined using a novel experimental and theoretical protocol. Our results show that mechanical loads decrease both actin-myosin attachment and detachment kinetics during actin sliding in skeletal and cardiac muscle, providing further insight into the mechanics of muscle contraction. | |
Item Format | ||
Item Language | English | |
Language | en_US | |
Rights | Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 United States | |
Rights URL | https://creativecommons.org/licenses/by-nc-nd/4.0/ | |
Title | The effects of force on actin-myosin ATPase activity | |
Type | Thesis | |
Rights Holder | Author(s) | |
Department | Biochemistry and Molecular Biology | |
Degree Level | Honors Thesis | |
Degree Name | Biochem and Molecular Biology | |
Degree Grantor | University of Nevada, Reno |