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Tangential Flow Filtration for the Purification of Lucentis®
The goal of the Genentech Capstone project is to design a process to manufacture and purify a “Lucentis®-like” (Lu-L) protein. This group has been working on the tangential flow filtration (TFF) portion of the project, which will be utilized multiple times throughout the separation and purification process. The primary purpose of tangential flow filtration is to exchange buffer solution. Exchanging buffer solution regulates the pH of the mixture in preparation for chromatography, and changes the mobile phase utilized in each different chromatography step. The secondary purpose is to remove impurities from the buffer, while keeping the desired product on the upstream side of the membrane. Finally, tangential flow filtration can be used to concentrate proteins, which is an important step in the creation of the Lu-L product, as it allows for a product with the necessary specifications of volume, concentration, and purity. The team conducted trials to concentrate the modeling analog, hemoglobin, to observe the anticipated behavior of the Lu-L protein throughout TFF. In addition to performing those trials, the as well as modeling the industrial scale up of the TFF process will prepare students to start working with the Genentech protein and create the optimized scale-up system for the Lu-L protein.